The jejunal mucosal scrapings were collected and immediately snap-frozen in liquid nitrogen and stored at  80°C.The QIAamp DNA stool Mini Kit (Qiagen, Hilden, Germany) was used to extract DNA from enteric bacteria injejunal mucosal scrapings and PCR amplification of theV3-V4 region (338F: ACTCCTACGGGAGGCAGCA,806R: GGACTACHVGGGTWTCTAAT) of 16S rDNA. Theamplification products were detected by 2% gel electrophoresis and PCR products were recovered by axyprepDNA gel extraction kit (Axygen, San Francisco, CA, USA).The PCR products were detected and quantified byQuantiFluorTM-ST microfluorometer (Promega, Madison,WI, USA) and then Miseq library was constructed forpyrosequencing on the Illumina MiSeq platform (Illumina,Madison, WI, USA). Clean sequences were assigned to
the same operational taxonomic units (OTUs) based on asimilarity ≥ 97%. Data were analysed on the free onlinecloud platform (https://cloud.majorbio.com/) of Majorbio(Shanghai, China). Bacterial species were further identi-fied by sequence comparison of OTUs on NCBI. The relative abundance of significantdifferences in phylum, genusand OTU levels was examined by the Kruskal–Wallis ranksum test. Spearman’s correlation analysis was furtheranalysed to uncover the relationship between jejunalmicrobiota and intestinal fat absorption.

感謝動(dòng)物營(yíng)養(yǎng)與飼料科學(xué)重點(diǎn)實(shí)驗(yàn)室（華東），農(nóng)業(yè)農(nóng)村部，浙江杭州 310058，中國(guó)。浙江省動(dòng)物飼料與營(yíng)養(yǎng)重點(diǎn)實(shí)驗(yàn)室，浙江杭州 310058，中國(guó)。浙江大學(xué)預(yù)防獸醫(yī)研究所，浙江杭州 310058，中國(guó)。6浙江省預(yù)防獸醫(yī)重點(diǎn)實(shí)驗(yàn)室，引用文獻(xiàn)！